Separation and Isolation of Fusion Protein using a New Native Preparative PAGE Device
نویسندگان
چکیده
منابع مشابه
Separation and isolation of fusion protein using a new native preparative PAGE device.
A human serum albumin and Thymosin α1 (HSA-Tα1) fusion protein was designed and over-expressed in Pichia pastoris. To purify the fusion protein, a new native preparative electrophoresis system that involved a modified device with a sample receiving chamber, and an assay method with Coomassie Blue G-250 tracing the collection of the protein of interest. In this device, two gels were run in paral...
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ژورنال
عنوان ژورنال: Journal of Chromatographic Science
سال: 2012
ISSN: 0021-9665,1945-239X
DOI: 10.1093/chromsci/bms077